FastQCFastQC Report
Fri 20 Jan 2023
US_Cal_Tuo_1_2013-05-14.trimmed1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUS_Cal_Tuo_1_2013-05-14.trimmed1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59275344
Sequences flagged as poor quality0
Sequence length75-92
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATT786970.13276515105504913No Hit
ATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAAC784040.13227084772380232No Hit
ATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCT720400.12153451188743838No Hit
AGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAACAT681390.11495336070930268No Hit
CATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAA675560.11396981517306758No Hit
ATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTT646390.10904871340771975No Hit
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG615110.10377164576219078No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)